제품설명
⊙ 제품 특장점
- 넓은 범위의 DNA 및 RNA input에서 높은 활성 유지 → 다양한 실험 응용 가능
- Standard 및 Rapid ligation buffer 포함 → 실험 최적화를 간편하게 수행
- 동급 성능을 제공하면서도 비용 절감 효과 → 경제적인 drop-in 솔루션
- 맞춤형 format 제공 → 고농축(high concentration) 버전 지원
- 엄격한 효소 제조 공정 → lots 간 일관된 성능 보장
⊙ Applications
- Cloning of restriction digestion and PCR products
- Joining of linkers or adapters to DNA
- Nick repair
- DNA self-circularization
⊙ QC Specifications
⊙ 제품특성
Unit definition: |
One unit is defined as the amount of DNA ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 20 μL 1X DNA Ligase Buffer following a 30-minute incubation at 23°C |
Reaction conditions: |
1X Rapid Ligation Buffer*/ Incubate at 20-25°C |
Storage buffer: |
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol pH 7.5 @ 25°C |
1X Rapid Ligation Buffer: | 6% PEG, 66 mM Tris-HCl, 10 mM MgCl₂, 1 mM DTT, 1 mM ATP pH 7.6 @ 25°C |
Heat inactivation: |
65°C for 10 min |
Molecular weight: |
55.3 kDa |
*Note: Other buffer formulations are also available
